Journal: RSC Advances

Article Title: Synthesis, radiolabeling, and biological evaluation of methyl 6-deoxy-6-[ 18 F]fluoro-4-thio-α- d -maltotrioside as a positron emission tomography bacterial imaging agent †

doi: 10.1039/d5ra00693g

Figure Lengend Snippet: Immunohistochemistry on tissue around the skin pocket. Expression of SGLT1 and SGLT2 in the mock-up area was evaluated by immunohistochemistry. (a) SGLT1 (red) was expressed on fibroblasts (green, vimentin-positive cells), which exhibited an increase in the inflammatory tissue. (b) Similarly, SGLT2 (red) was expressed on fibroblasts (green). The scale bars represent 40 μm.

Article Snippet: To assess cells expressing sodium glucose co-transporter1 (SGLT1) and SGLT2, the samples were stained with rabbit anti-SGLT1 antibody (NBP2-20338, Novus, 1 : 100) or rabbit anti-SGLT2 antibody (GTX59872, GeneTex, 1 : 100).

Techniques: Immunohistochemistry, Expressing

Journal: RSC Advances

Article Title: Synthesis, radiolabeling, and biological evaluation of methyl 6-deoxy-6-[ 18 F]fluoro-4-thio-α- d -maltotrioside as a positron emission tomography bacterial imaging agent †

doi: 10.1039/d5ra00693g

Figure Lengend Snippet: Expression of SGLT1 and SGLT2 in the skin pocket from the control group and from the non-infectious inflammation group was quantified with qPCR. The expression of the target genes is indicated as the ratio to β-actin, a house keeping gene. Expression of SGLT1 in the non-infectious inflammation group was about 12 times that of the control group. Expression of SGLT2 in the non-infectious inflammation group was about 1.8 times the control group.

Article Snippet: To assess cells expressing sodium glucose co-transporter1 (SGLT1) and SGLT2, the samples were stained with rabbit anti-SGLT1 antibody (NBP2-20338, Novus, 1 : 100) or rabbit anti-SGLT2 antibody (GTX59872, GeneTex, 1 : 100).

Techniques: Expressing, Control, Gene Expression

Journal: RSC Advances

Article Title: Synthesis, radiolabeling, and biological evaluation of methyl 6-deoxy-6-[ 18 F]fluoro-4-thio-α- d -maltotrioside as a positron emission tomography bacterial imaging agent †

doi: 10.1039/d5ra00693g

Figure Lengend Snippet: Accumulation of [ 18 F]MFTMT and derivative of [ 18 F]MFTMT by SGLT1. (a) Stable expression of human SGLT1 (hSGLT1) or SGLT2 (hSGLT2) was established in CHO-K1 cells. CHO-K1-hSGLT1 and CHO-K1 hSGLT2 cells internalized 1-NBDG, a specific substrate for SGLT1 and SGLT2. Scale bars represent 100 μm. (b) Retention of [ 18 F]MFTMT and (c) retention of [ 18 F]MFTM by CHO-K1 cells overexpressing SGLTs. CHO-K1-hSGLT1, hSGLT2, and control cells were incubated with 20 μCi mL −1 of [ 18 F]MFTMT for 1 h, and the remaining radioactivity was evaluated. Some cells were treated with vehicle, mizagliflozin, a specific SGLT1 inhibitor, or dapagliflozin, a specific SGLT2 inhibitor. Retention of both [ 18 F]MFTMT and [18F]MFTM in CHO-K1-hSGLT1 cells were significantly higher compared to CHO-K1-hSGLT2 and control cells, and mizagliflozin significantly suppressed the retention of [ 18 F]MFTMT and [ 18 F]MFTM.

Article Snippet: To assess cells expressing sodium glucose co-transporter1 (SGLT1) and SGLT2, the samples were stained with rabbit anti-SGLT1 antibody (NBP2-20338, Novus, 1 : 100) or rabbit anti-SGLT2 antibody (GTX59872, GeneTex, 1 : 100).

Techniques: Expressing, Control, Incubation, Radioactivity